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affinityscript qpcr cdna synthesis kit  (Agilent technologies)


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    Structured Review

    Agilent technologies affinityscript qpcr cdna synthesis kit
    Affinityscript Qpcr Cdna Synthesis Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/affinityscript+qpcr+cdna+synthesis+kits/pm39142076-80-22-28
    Average 90 stars, based on 1 article reviews
    affinityscript qpcr cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Pathophysiological response in experimental trauma-related acute kidney injury
    Article Snippet: RNA was isolated from snap-frozen mouse kidneys using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using AffinityScript qPCR cDNA Synthesis Kits (Agilent Technologies, Waldbronn, Germany) and RT-qPCR was carried out employing the Brilliant III Ultra-Fast SYBR® Green Master Mix (Agilent Technologies). ..

    Article Title: Role of the transgenic human thyrotropin receptor A-subunit in thyroiditis induced by A-subunit immunization and regulatory T cell depletion
    Article Snippet: Real-time PCR for mouse TSHR and human A-subunit in thyroid tissue Thyroids from A-subunit transgenics (low-expressor 51·9 and high-expressor 50·6 lines) and WT littermates (four per strain) were dissected from surrounding tissue, diced into small pieces and transferred to RNAlater (Qiagen, Valencia, CA, USA) for storage at 4°C. mRNA was isolated for individual thyroids using Oligotex mRNA mini kits (Qiagen), treated with Turbo DNase (Applied Biosystems/Ambion, Austin, TX, USA), precipitated with potassium acetate, glycogen and ethanol, washed and resuspended in RNAse-free water. .. Reverse transcription (RT) into cDNA was performed using oligo-dT and AffinityScript QPCR cDNA synthesis kits (Stratagene, La Jolla, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Pathophysiological response in experimental trauma-related acute kidney injury
    Article Snippet: RNA was isolated from snap-frozen mouse kidneys using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using AffinityScript qPCR cDNA Synthesis Kits (Agilent Technologies, Waldbronn, Germany) and RT-qPCR was carried out employing the Brilliant III Ultra-Fast SYBR® Green Master Mix (Agilent Technologies). ..

    cDNA Synthesis:

    Article Title: Pathophysiological response in experimental trauma-related acute kidney injury
    Article Snippet: RNA was isolated from snap-frozen mouse kidneys using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using AffinityScript qPCR cDNA Synthesis Kits (Agilent Technologies, Waldbronn, Germany) and RT-qPCR was carried out employing the Brilliant III Ultra-Fast SYBR® Green Master Mix (Agilent Technologies). ..

    Article Title: Role of the transgenic human thyrotropin receptor A-subunit in thyroiditis induced by A-subunit immunization and regulatory T cell depletion
    Article Snippet: Real-time PCR for mouse TSHR and human A-subunit in thyroid tissue Thyroids from A-subunit transgenics (low-expressor 51·9 and high-expressor 50·6 lines) and WT littermates (four per strain) were dissected from surrounding tissue, diced into small pieces and transferred to RNAlater (Qiagen, Valencia, CA, USA) for storage at 4°C. mRNA was isolated for individual thyroids using Oligotex mRNA mini kits (Qiagen), treated with Turbo DNase (Applied Biosystems/Ambion, Austin, TX, USA), precipitated with potassium acetate, glycogen and ethanol, washed and resuspended in RNAse-free water. .. Reverse transcription (RT) into cDNA was performed using oligo-dT and AffinityScript QPCR cDNA synthesis kits (Stratagene, La Jolla, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: Pathophysiological response in experimental trauma-related acute kidney injury
    Article Snippet: RNA was isolated from snap-frozen mouse kidneys using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using AffinityScript qPCR cDNA Synthesis Kits (Agilent Technologies, Waldbronn, Germany) and RT-qPCR was carried out employing the Brilliant III Ultra-Fast SYBR® Green Master Mix (Agilent Technologies). ..

    SYBR Green Assay:

    Article Title: Pathophysiological response in experimental trauma-related acute kidney injury
    Article Snippet: RNA was isolated from snap-frozen mouse kidneys using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using AffinityScript qPCR cDNA Synthesis Kits (Agilent Technologies, Waldbronn, Germany) and RT-qPCR was carried out employing the Brilliant III Ultra-Fast SYBR® Green Master Mix (Agilent Technologies). ..



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